Biophysics

The role of polyproline in the membrane translocation of arginine-rich cell-penetrating peptides: A study based on molecular dynamics simulations

Recent experimental studies have demonstrated that incorporating polyproline segments into arginine (R)‑rich cell‑penetrating peptides (CPPs) enhances their membrane penetration efficiency. To investigate this effect, we designed a …

Spontaneous translocation of carbon nanotubes across a model membrane

Carbon nanotubes (CNTs) have been utilized in various applications, including drug delivery and energy conversion. It is essential to elucidate the underlying transport mechanism across cell membranes to understand their role as drug-delivery agents. …

Insights into translocation of arginine-rich cell-penetrating peptides across a model membrane

It is well-known that membrane deformation and water pores contribute to the spontaneous translocation of arginine-rich cell-penetrating peptides (CPPs). We confirm this through the observation of the spontaneous translocation of single R9 …

Spontaneous translocation of cell-penetrating peptides across a model membrane using molecular dynamics simulations

Observing the spontaneous translocation of cell-penetrating peptides across a lipid bilayer in molecular dynamics simulations is challenging. However, in this study, we have successfully demonstrated the spontaneous translocation of Arg9 …

Spontaneous translocation of cell-penetrating peptides using the weighted ensemble method within molecular dynamics simulations

Our findings demonstrate the occurrence of self-induced translocation of a specific cell-penetrating peptide (CPP) across a model membrane through the utilization of the weighted ensemble (WE) approach. Furthermore, our investigation highlights the …

External electric field effect on the translocation of R-derivatives peptides across a symmetric lipid bilayer using umbrella sampling

Translating cargoes like nanoparticles (NP) and macromolecules like DNA and RNA across the model membrane through different cell-penetrating peptides (CPPs) has been the point of research for the last few years. Electroporation is a technique to …

Translocation of cell-penetrating peptides using the weighted ensemble method within molecular dynamics simulations approach

We report spontaneous translocation of a single cell-penetrating peptide (CPP) across a model membrane using the weighted ensemble (WE) method within the molecular dynamics (MD) simulations approach. Furthermore, we show that a water pore plays a …

Translocation of a single Arg9 peptide across a DOPC/DOPG(4:1) model membrane using the weighted ensemble method

It is difficult to observe a spontaneous translocation of cell-penetrating peptides(CPPs) within a short time scale (e.g., a few hundred ns) in all-atom molecular dynamics(MD) simulations because the time required for the translocation of usual CPPs …

Translocation of cell-penetrating peptides across a model membrane using molecular dynamics simulations

In this study, we report a single Arg9(R9) peptide translocation across a DOPC/DOPG(4:1) model membrane within an order of a few tens ns scale by using the weighted ensemble(WE) method. We identify how water molecules play a role during the …

Translocation of a single Arg9 peptide across a DOPC/DOPG(4:1) model membrane using the weighted ensemble method

A spontaneous translocation of cell-penetrating peptides(CPPs) in all-atom molecular dynamics(MD) simulations is not expected within a short time scale (e.g., a few hundred ns) because it is believed that the time required for the translocation of …